C2548-80T
CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 antigen, 5F7, Blood-brain barrier HT7 antigen, Collagenase stimulatory factor, Emmprin, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, TCSF, Tumor cell derived collagenase stimulatory factor)
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£479.00
- SKU:
- C2548-80T
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human T cell line Molt 13
- Klon:
- 7H225 (UM-8D6)
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml Gentamicin.
- Weitere Details:
- Human CD147 is a transmembrane glycoprotein expressed more intensely on thymocytes than on mature peripheral blood T cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml labels 5x10e5 cultured human Raji cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human Raji cells were washed and incubated for 45 minutes on ice with 80ul of C2548-80T at 10ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat x mouse. 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice