C2548-41-FITC
CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (FITC)
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£960.00
- SKU:
- C2548-41-FITC
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Murine CD137 human Fc fusion protein.
- Klon:
- LOB12
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- A glycoprotein (256 amino acids) of 30kD, which exists as both a monomer and a 55kD dimer on the surface of activated T cells is identified as 4-1BB (also known as CD137). 4-1BB is an inducible receptor-like protein expressed on the cell surface of activated splenic T cells and thymocytes. 4-1BB is structurally related to the members of NGFR/TNFR superfamily which are characterized by the presence of three-six patterns of a cysteine-rich motif in their extracellular domains. Other members of this family include low affinity NGFR, two receptors for TNF (TNFR-I & TNFR-II), CD30, CD40, OX40, Fas, and CD27. These molecules are involved in cell growth, survival, and death processes. The cytoplasmic domain of 4-1BB include two runs of acidic amino acids, a potential p56lck binding site, five consecutive glycines at the C-terminus, and four potential phosphorylation sites: one tyrosine, two threonine, and one serine. The cytoplasmic domain of 4–1BB displays sequence homology to CD4 and CD8 and is responsible for the binding of 4–1BB to p56lck, a protein tyrosine kinase involved in T cell proliferation. Applications: Flow Cytometry: 1:10-1:50. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Recommended Secondary Antibody: F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) Recommended Negative Controls: Rat IgG2a Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice