C2548-41-BIOTIN
CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (Biotin)
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£960.00
- SKU:
- C2548-41-BIOTIN
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Murine CD137 human Fc fusion protein.
- Klon:
- LOB12
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Weitere Details:
- A glycoprotein (256 amino acids) of 30kD, which exists as both a monomer and a 55kD dimer on the surface of activated T cells is identified as 4-1BB (also known as CD137). 4-1BB is an inducible receptor-like protein expressed on the cell surface of activated splenic T cells and thymocytes. 4-1BB is structurally related to the members of NGFR/TNFR superfamily which are characterized by the presence of three-six patterns of a cysteine-rich motif in their extracellular domains. Other members of this family include low affinity NGFR, two receptors for TNF (TNFR-I & TNFR-II), CD30, CD40, OX40, Fas, and CD27. These molecules are involved in cell growth, survival, and death processes. The cytoplasmic domain of 4-1BB include two runs of acidic amino acids, a potential p56lck binding site, five consecutive glycines at the C-terminus, and four potential phosphorylation sites: one tyrosine, two threonine, and one serine. The cytoplasmic domain of 4–1BB displays sequence homology to CD4 and CD8 and is responsible for the binding of 4–1BB to p56lck, a protein tyrosine kinase involved in T cell proliferation. Applications: Flow Cytometry: 1:10-1:50. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Recommended Secondary Antibody: F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) Recommended Negative Controls: Rat IgG2a Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice