CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (Biotin)

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£1,042.00

SKU:: C2548-40F
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Leu24-Gln186 from human CD137, expressed in NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Reconstitute with 250ul sterile PBS.
Weitere Details:: 4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The human and mouse proteins share 60% amino acid sequence identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells using PHA-treated human T cells ELISA (Capture): 0.2-0.8ug/ml ELISA (Detection): 0.1-0.4ug/ml Western Blot: 0.1ug/ml Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood mononuclear cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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