C2548-39L
CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (PE)
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£735.00
- SKU:
- C2548-39L
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Ectodomain of recombinant human 4-1BB fusion protein
- Klon:
- 7H126 (4B4-1)
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 0.2% BSA, 500mM potassium chloride, 150mM sodium chloride, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50 (1.6ul/5x10e5 cells) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human CEM cells were washed and incubated 45 minutes on ice with 80ul of C2548-39L at 1:50 dilution (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.12 log10 fluorescent units when compared to a Mouse IgG1/PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of C2548-39F. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice