C2548-39H

CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (Biotin)

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£639.00

SKU:: C2548-39H
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Ectodomain of recombinant human 4-1BB fusion protein
Klon:: 7H126
Format:: Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Weitere Details:: Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival. Applications: Suitable for use in Flow Cytometry andiscontinued ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured human CEM were stimulated 1 day in the presence of PMA (10ng/ml) and ionomycin (1uM). Five x 10e5 Cells were washed and incubated 45 minutes on ice with 80ul of C2548-39H at 10ug/ml. 2. Cells were washed twice and incubated with 2º reagent Streptavidin/R-Phoycoerythrin (S7973-88P). 3. Cells washed three times, fixed and analyzed by FACS. 4. A net 69% sub population of the cells stained positive with a mean shift of 2.0 log10 fluorescent units when compared to a mouse IgG1/Biotin negative control at a similar concentration. 5. Binding was blocked when cells were pre-incubated 10 min. with 20ul of C2548-39F at 0.5mg/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice