C2515-40J
CD252 (Tumor Necrosis Factor Ligand Superfamily Member 4, Glycoprotein Gp34, OX40 Ligand, OX40L, TAX Transcriptionally-activated Glycoprotein 1, TNFSF4, TXGP1) (APC)
Größe
£889.00
- SKU:
- C2515-40J
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography from cell culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln51-Leu183 from human CD252, expressed in NS0 cells.
- Klon:
- 10K21
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Weitere Details:
- OX40 Ligand, also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily and is now designated TNFSF4. Engagement of OX40 Ligand by OX40 (CD134) on activated CD4T cells promotes the proliferation and differentiation of activated B cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells Optimal dilutions to be determined by the researcher. Flow Cytometry: This antibody has been tested for flow cytometry using stimulated peripheral blood mononuclear cells (PBMCs). 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, 10ul of conjugated antibody was added to up to 1x10e6 cells and incubated for 30 minutes at RT. 3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. The cells were resuspended in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG 1 antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice