C2515-19A
CD134 (ACT35 Antigen, OX40, OX40L Receptor, TAX Transcriptionally-activated Glycoprotein 1 Receptor, TXGP1L, Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4) (Biotin)
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£639.00
- SKU:
- C2515-19A
- Reinheit:
- >95% SDS-PAGE. Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HUT 102 T cells.
- Klon:
- 7H163
- Format:
- Supplied as a liquid in PBS, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with biotin.
- Weitere Details:
- Tumor necrosis factor receptor superfamily member 4 (OX40, CD134) is a membrane receptor found on activated CD4+ and CD8+ T cells and B cells (1). OX40 binds to OX40 ligand (OX40L) and other TNF receptor-associated factors (TNAF2, TRAF3, TRAF5). OX40-OX40L complex produces CD28 independent co-stimulatory signals. OX40 is involved in the pathologic cytokine storm associated in certain viral infection, such as H4N1 bird flu. OX40 starts a survival signal to the T-cell preventing activation-induced apoptosis, but up regulates cytokine production. Inhibition of OX40 using OX40-immunoglobulin has been linked to reduction of immune overreaction without affecting the viral clearance (2). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 5x10e5 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of C2515-19A at a concentration of 10ug/ml. Cells were washed twice and incubated with S7973-88P, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.66 log 10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cell were pre incubated with 20ul of 0.5mg/ml C2515-19. Recommended Negative Control: I1904-78R2: IgG1 Murine Negative Control (Biotin) Recommended Blocking Antibody: C2515-19: CD134 (OX40). Recommended Secondary Reagent: S7973-88P: Streptavidin/R-Phycoerythrin Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice