C2464-05B
CD130 (Interleukin 6 Receptor beta, gp130, neutralizing, IL-6Rb, Interleukin-6 signal transducer)
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£863.00
- SKU:
- C2464-05B
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.1EU/1ug (LAL)
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gln23-Glu617 of mouse CD130 expressed in NS0 cells.
- Klon:
- 9H92
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component specific to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low affinity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. CD126 is single chain type I transmembrane protein with Mr=130–140kD and is a common receptor subunit for many cytokines, forming complexes with CD126, IL-11R, LIF-R, and other proteins. These gp130-containing complexes mediate signaling of oncostatin M, IL-6, IL-11, LIF, CNF, and cardiolipotrophin 1. gp130 is expressed at low levels by most cell types. This antibody was recognized as reacting with gp130 at the Sixth Leukocyte Typing Workshop. Applications: Suitable for use in Neutralization and ELISA. Other applications not tested. Recommended Dilutions: ELISA (Capture): 2-8ug/ml Neutralization: Measured by it’s ability to neutralize IL-6 mediated proliferation of the 7TD1 mouse hybridoma line. The ND50 is typically 0.15-1ug/ml in the presence of 25pg/ml recombinant human IL-6. Optimal dilutions to be determined by the researcher. Matched Pair: Capture: C2464-05B Detection: 052558 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice