C2448-39
CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)
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£1,792.00
- SKU:
- C2448-39
- Anwendung:
- IHC-Fr
- Reinheit:
- Supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Klon:
- YR145
- Format:
- Supplied as a liquid in Tris Buffer, pH 7.3-7.7, 1% BSA, 0.09% sodium azide
- Weitere Details:
- CD117, c-kit is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium and mast cells. This receptor is found on a wide variety of tumor cells (follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, breast; malignant melanoma, endodermal sinus tumor, and small cell carcinoma) but has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi’s sarcoma, and tumors of smooth muscle origin. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin/frozen): 1:25-1:100. Optimal dilutions to be determined by the researcher. Immunohistochemistry Protocol: 1. Cut 3-4um sections of formalin-fixed paraffin embedded tissue and place on positively charged slides; dry overnight at 58ºC. 2. Deparaffinize, rehydrate and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjugation with a pressure cooker. The preferred method allows for stimultaneous deparaffinization, rehydration and epitope retrieval. Upon completion, rinse with 5 changes of ddH2O. 3. If using a HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Breast, GIST, Skin, Testes Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice