C2445-30L
CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2, IgSF2, Immunoglobulin superfamily member 2, LOC381460) (Trehalose & azide free)
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£230.00
- SKU:
- C2445-30L
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- rmIGSF2 extracellular domian
- Klon:
- 7H22
- Format:
- Supplied as a powder lyophilized from a solution containing PBS. No stabilizing protein and no preservative added.
- Weitere Details:
- IGSF2, also known as CD101, is an Ig-superfamily transmembrane protein. It is expressed on dendritic cells, Langerhans cells, granulocytes, and activated T cells. IGSF2 ligation is involved in T cell activation. Polymorphisms in IGSF2 are associated with susceptibility to type I diabetes. The extracellular domains of mouse and human IGSF2 share 70% amino acid sequence identity. Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified recombinant mouse IGSF2 (rmIGSF2; Accession # BAD97674) extracellular domain. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice