C2445-30D
CD101 (IgSF2, EWI-101, Gm1016, Glu-Trp-Ile EWI Motif-Containing Protein 101, Gm734, Immunoglobulin Superfamily, Member 2) (APC)
Größe
£754.00
- SKU:
- C2445-30D
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gln21-Tyr1033 from mouse CD101 expressed in CHO cells.
- Klon:
- 7H22
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Weitere Details:
- IGSF2, also known as CD101 and V7 is a 135-140kD member of the EWI family, Ig superfamily of molecules. It is a type I transmembrane glycoprotein that is expressed on dermal dendritic cells (DCs), granulocytes, monocytes, activated T cells, and Tregs. IGSF2 ligation is involved in T cell activation. On dermal DCs, CD101 ligation induces IL-10 expression, and on activated T cells, CD101 ligation blocks IL-2 expression, two effects that downregulate T cell activity. Notably, in mouse, CD101 expression on CD62L++ Tregs identifies a population of cells that have potent suppressor activity. The extracellular domain of mouse shares 86% and 69% amino acid sequence identity with the ECD of rat and human CD101, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice