C2442-07B-ML550

CD94 (CD94 Antigen, Killer Cell Lectin-like Receptor Subfamily D Member 1, KLRD1, KLRD1 Protein, Kp43, Natural Killer Cells Antigen CD94, NK Cell Receptor) (MaxLight 550)

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£837.00

SKU:: C2442-07B-ML550
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Rat
Immunogen:: CHO cells transfected with B6 allele of CD94.
Klon:: 18d3
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Weitere Details:: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The murine CD94 molecule forms heterodimers with NKG2A, C or E subunits to form the CD94/NKG2 receptor. In mice the CD94/NKG2 receptor recognizes Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life. N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors (1). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:200, Use 10ul to label 10e6 cells in 100ul. Optimal dilution determined by the researcher Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice