C2441-85B-APC
CD90.2 (Thy1.2, Thy 1b) (APC)
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£960.00
- SKU:
- C2441-85B-APC
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- EL-4 cells.
- Klon:
- FF-10
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Weitere Details:
- Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta (θ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). The human homolog was isolated in 1980 as a 25kDa protein (p25) of T-lymphoblastoid cell line MOLT-3 binding with anti-monkey-thymocyte antisera. The conserved gene and its alleles Thy-1 has been conserved throughout vertebrate evolution and even in some invertebrates, with homologs described in many species like squid, frogs, chickens, mice, rats, dogs, and humans. The Thy-1 gene is located at human chromosome 11q22.3 (mouse chromosome 9qA5.1). Some believe that there may be a functional significance of both this gene and CD3 delta subunit (T3D) mapping to chromosome 11q in man and chromosome 9 in mouse, though there is no homology (in fact this speculation lead to its localization in chromosome 11q-the human chromosome region syntenic to mouse chromosome 9 which harbored T3D). In mouse there are two alleles Thy1.1 (Thy 1a, CD90.1) and Thy1.2 (Thy 1b, CD90.2) which differ by one amino acid at position 108 (arginine in Thy-1.1 and glutamine in Thy-1.2). Thy 1.2 is expressed by most strains of mice, whereas Thy1.1 is expressed by some like AKR/J and PL mouse strains. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Sprague-Dawley rats were fused with cells from the mouse SP2/0-Ag14 myeloma cell line. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice