C2433-10D
CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (PE)
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£704.00
- SKU:
- C2433-10D
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human B cell line from Burkitt lymphoma
- Klon:
- 7H100
- Format:
- Supplied as a liquid in 50mM PBS, pH 7.5, 0.2% BSA, 500mM potassium chloride, 150mM sodium chloride, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion, and cellular activation (1). Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation (2, 3). Recent work with CD81-null mice indicates CD81 is important for development of humoral immune response (4). Applications: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 labels 5x10e5 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Raji cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2433-10D at a dilution factor of 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 2.11 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was blocked when cells were preincubated for 10 minutes with 20ul of 0.5mg/ml C2433-10A. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice