C2425-01LX
CD70 (CD70 Antigen, CD27 Ligand, CD27L, CD27-L, CD27LG, Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7)
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£479.00
- SKU:
- C2425-01LX
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human WM-1 (Waldenström's macroglobulinemia) cell line
- Klon:
- 7H173 (BU69)
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
- Weitere Details:
- Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml (80ul) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2425-01LX at a concentration of 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent goat anti-mouse IgG (FITC), after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.30 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice