C2425-01B
CD70 (CD27L, CD27-L, CD27LG, CD27 Ligand, CD70 Antigen, TNFSF7, Tumor Necrosis Factor Ligand Superfamily Member 7)
Größe
£856.00
- SKU:
- C2425-01B
- Reinheit:
- Purified by immunoaffinity chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Gln45-Pro193 from human CD70 Ligand, expressed in NS0 cells.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- Human CD27 Ligand (also CD70) is a 30kD type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20aa cytoplasmic region and a 155aa extracellular domain that shows multiple b-strands. It is an inducible trimer that occurs on B cells, T cells and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using THP-1 human acute monocytic leukemia cell line Flow Cytometry: 2.5ug labels 10e6 Raji cells from human Burkitt’s lymphoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice