C2424-01B
CD69 (FITC)
Größe
£557.00
- SKU:
- C2424-01B
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- IL-2 activated human NK cells
- Klon:
- 7H192
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC). FITC:IgG ratio: 8:1.
- Weitere Details:
- Human CD69 is expressed by platelets and mature thymocytes and appears rapidly after activation on T cells, B cells and NK cells. This pattern of cellular CD69 expression has suggested a lymphocyte activation role. Functional studies with antibodies to CD69 also support an activation role for CD69. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (10ug/ml) labels 5x10e5 cultured human HEL92.1 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human HEL92.1 cells were washed and pre-incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. Cells were incubate for 45 minutes on ice with 80ul of C2424-01B at a 1:50 dilution factor (10ug/ml). 3. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a mouse IgG2a/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD69 antibody (C2424-01). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
- Versandbedingungen:
- Blue Ice