C2418-02E
CD63 (CD63 antigen, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, Melanoma-associated antigen ME491, MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30, Tspan-30) (FITC)
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£557.00
- SKU:
- C2418-02E
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human eosinophils
- Klon:
- AHN16.1/46-4-5
- Format:
- Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with FITC. FITC/IgG molar ratio =7.4
- Weitere Details:
- Human CD63 is expressed on activated platelets and also on monocytes and macrophages. CD63 contains four hydrophobic transmembrane regions and is a member of the tetraspan family. CD63 appears to be involved with cell adhesion and associates with VLA-3 and VLA-6 integrins. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (or use 1.6ul concentrated stock per 5x10e5 cells) Optimal dilutions to be determined by the researcher. Performance: 5x10e5 cultured HPB-MLT cells were washed and pre incubated 5 minutes with 20ul of human IgG at 250ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of C2418-02E at a 1:50 dilution factor (10ug/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.12 log10 fluorescent units when compared to a mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD63 antibody (Cat. #C2418-02C). Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice