C2410-62B
CD56 (MSK39, NCAM, NCAM-1, N-CAM-1, Neural cell adhesion molecule 1)
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£479.00
- SKU:
- C2410-62B
- Reinheit:
- Purified from culture supernatant by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to soluble human CD56 ( first four Ig-like domains)
- Klon:
- ANC7C7
- Format:
- Supplied as a liquid in PBS, pH 7.5.
- Weitere Details:
- Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 Ficoll-prepared human peripheral blood lymphocytes were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD56 antibody at 10ug/ml. Cells were washed twice and incubated with goat anti-mouse IgG-FITC, after which they were washed three times, fixed and analyzed by FACS. A net 19% sub-population of the cells stained positive with a mean shift of 1.18 log10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice