C2410-02A-BIOTIN
CD55 (DAF, Decay Accelerating Factor) (Biotin)
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£940.00
- SKU:
- C2410-02A-BIOTIN
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human fibroblast cell line
- Klon:
- 3H1564 (BRIC 216)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Weitere Details:
- CD55 is a 70kD glycoprotein also known as Decay Accelerating Factor (DAF). Distributed on erythrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. Found specifically at the foetal-maternal interfaces in placenta.CD55 has reduced expression on individuals with paroxysmal nocturnal haemoglobinuria. This antibody has a functional binding affinity to erythrocytes of 8.7x10e7 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Functional assays. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Functional assays: Removal of sodium azide is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice