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  2. Monoclonal

C2409-48G-ML490

CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (MaxLight 490)

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£852.00

SKU:
C2409-48G-ML490
Reinheit:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Lagerbedingungen:
4°C Do Not Freeze
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Rat Ax cells (a HEV derived cell line).
Klon:
1A29
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Weitere Details:
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation. CD29 is reported to inhibit beta 1 integrin mediated adhesion. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 1:25-1:50. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: frozen Optimal dilution determined by the researcher Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells from the PAI mouse myeloma cell line. Recommended Negative Controls: I1904-78Y: IgG1, Murine Negative Control (For rat tissue) Recommended secondary antibodies: (Rat Adsorbed) I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Versandbedingungen:
Blue Ice