C2404-08D-ML650
CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a) (MaxLight 650)
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£1,070.00
- SKU:
- C2404-08D-ML650
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human squamous cell carcinoma cells (SCC9).
- Klon:
- 2Q995 (ASC-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The a3 subunit, also known as CD49c and VLA-3 a subunit, forms a non-covalent heterodimer with the Integrin b1 subunit (CD29). Integrin a3b1 is a receptor for laminin, fibronectin, collagen, epiligrin, and thrombospondin. Applications: Suitable for use in Inhibition of Cell Attachment, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin, and laminin GD-2 peptide. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit cell adhesion to fibronectin or collagen type IV. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Immunoprecipitation: Precipitates PAGE bands of 130kD (reduced) or 116kD and 135kD (non-reduced) from surface-biotinylated SCC9 or SKOV-3 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice