C2404-06N
CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B) (FITC)
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£620.00
- SKU:
- C2404-06N
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- RIL-2 propogated NK1.1+ cells from C57BL/6 mice.
- Klon:
- DX5
- Format:
- Supplied as a liquid in 0.09% sodium azide, 0.5% BSA. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines. CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets. Applications: Suitable for use in Flow Cytometry. Not suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Recommended Negative Controls: Mouse IgG1 Negative Control Fusion Partners: Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice