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C2404-06F-PE

CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B) (PE)

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£940.00

SKU:
C2404-06F-PE
Reinheit:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Lagerbedingungen:
4°C Do Not Freeze
Hersteller:
United States Biological
Host:
Mouse
Immunogen:
Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
Klon:
3H1472 (16B4)
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Weitere Details:
Human CD49b is an integrin alpha 2 subunit that forms a heterodimer with beta 1 integrin and functions as an adhesion molecule. Applications: Suitable for use in Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Functional Assays. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Use sodium citrate buffer, pH 6, for heat mediated antigen retrieval. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher Recommended Secondary Antibodies (FC): I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab Rb x Mo Recommended Secondary Antibodies (IHC): I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb x Mo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Versandbedingungen:
Blue Ice