C2404-06E
CD49b (Integrin alpha2, ITGA2, alpha-2 subunit of VLA-2 Receptor, Platelet Antigen B)
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£691.00
- SKU:
- C2404-06E
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
- Klon:
- 3H1471 (31H4)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Weitere Details:
- Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing alpha integrin 2 combines with beta integrin 1 to form a collagen-binding integrin referred to as glycoprotein Ia/IIa (gpIa/IIa) when expressed on platelets, and very late (activation) antigen 2 ('VLA-2') when found on T-cells. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. CD49b is expressed approximately 50% of monocytes and most adherent cell lines. CD49b forms a non-covalent association with the 130kD VLA beta chain (CD29) in the VLA-2 complex. The CD49b/CD29 complex is also known as a2/b1 and VLA-2. The complex mediates binding of cells to collagen (types I, II, III, and IV) and to laminin. The complex of CD49b associated with CD29 (integrin b1) is a receptor for laminin and collagen. This complex is expressed by some CD4-positive T-cells, natural killer cells, epithelial cells and platelets. Applications: Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 10ug/ml Immunoprecipitation: 25-50ug/ml Flow Cytometry: 1:25-1:50 Optimal working dilutions to be determined by researcher. Recommended Secondary Antibodies: I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice