C2399-17
CD45 (Leukocyte Common Antigen, LCA, LY-5 T200)
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£460.00
- SKU:
- C2399-17
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Klon:
- 5B23 (ICO-59)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added.
- Weitere Details:
- CD45 or Leukocyte Common Antigen (LCA/T200) family is comprised of at least four isoforms (CD45RA, CD45RB, CD45RC, and CD45RO) of membrane glycoproteins with MW of 180kD-220kD. It consists of a large, heavily glycosylated, amino-terminal extracellular domain of 400-550 aa, a single transmembrane region and a large carboxy-terminal cytoplasmic domain of 700aa. CD45/LCA is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of CD45 proteins has phosphatase activity and is involved in regulation of transmembrane signals, functioning as a leukocyte membrane receptor capable of transducing signals via its phosphotyrosine phosphatase domains. Several studies on the function of CD45 in leukocytes support a crucial role of CD45 in the regulation of leukocyte activation, development, and function. In these processes, distinct CD45 isoforms appear to play differential functions. The diversity of the structure, size, and carbohydrate content of different CD45 isoforms is cell-type dependent and developmentally regulated. This structural heterogeneity is mainly the result of the alternative splicing of a single CD45 gene. Applications: Suitable for use in ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice