C2397-76W

CD43 (CD43 Antigen, Galactoglycoprotein, GALGP, GPL115, Leukocyte Sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN)

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£460.00

SKU:: C2397-76W
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Mouse WEHI 274.3 myeloid tumor cells
Klon:: 1B11
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Weitere Details:: CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It does not present on resting B cells and erythrocytes. While the 130kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. This antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by this monoclonal antibody is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤1ug/10e6 cells in 100ul volume Immunohistochemistry: acetone-fixed frozen sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice