C2394-12B
CD41 (Integrin alpha IIb chain, GpIIb-IIIa, Platelet Membrane gpIIb-IIIa Complex)
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£678.00
- SKU:
- C2394-12B
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- 10e9 human platelets suspension. The supernatant fluid from postitive clones was screened by ELISA coated with washed platelets
- Klon:
- 474
- Format:
- Supplied as a lyophilized powder in 0.01M PBS pH 7.2. Reconstitute with sterile ddH2O to ~1mg/ml.
- Weitere Details:
- Human platelet membrane glycoprotein Glycoprotein IIb/IIIa (GP IIb/IIIa) is a dominate receptor on the platelet membrane with very high molecular weight (>200kD). After the platelet is activated, the GP IIb/IIIa receptor complex changes conformation and binds to fibrinogen in blood circulation with high affinity. The activation of platelets is initiated locally when the endothelial layer is wounded and the platelets are exposed to the collagen underneath the endothelium. The fibrinogen aggregates with platelets when binding to the GP IIb/IIIa receptor on platelets, thus primary haemostasis (Platelet clot) is formed. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 10-20ug/ml (culture supernatants) will allow visualization of NP 40 solubized human normal platelets. Under reducing conditions they will bind against the human platelet membrane glycoproteins IIb/IIIa complex, which have a molecular weights of ~130,000 (IIb) and 115,000 (IIIa). The titer of purified ascites was determined by ELISA coated with 100ul of 1x10e8 washed platelets Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice