C2391-01A

CD39 (Apyrase, ATPDase, EC3.6.1.5, Ecto-Apyrase,Ecto-ATPase, ENTPD1, Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase-1, Nucleoside triphosphate diphosphohydrolase 1, Vascular ATP diphosphohydrolase)

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£479.00

SKU:: C2391-01A
Reinheit:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Human WM-1 (Waldenström's macroglobulinemia) cell line
Klon:: 7H171
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
Weitere Details:: Human CD39 is found on most mature B cells, activated NK cells and activated T cells, as well as Neutrophills and Monocytes. CD39 has homology to the N-myc family of proteins and has been cloned. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml labels 5x10e5 ficoll prepared human peripheral blood lymphocyte cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared human peripheral blood lymphocytes cells were washed and preincubated ~5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80ul of C2391-01A at 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse after which they were washed three times, fixed and analyzed by FACS. 3. A net 19.1% sub population of the cells stained positive with a mean shift of 1.02 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice