C2389-01D
CD37 (GP52-40, Leukocyte antigen CD37, MGC120234, Tetraspanin-26, TSPAN26, Tspan-26)
Größe
£479.00
- SKU:
- C2389-01D
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human spleen cells from a patient with hairy cell leukemia
- Klon:
- IPO-24
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride.
- Weitere Details:
- Human CD37 is a member of the tetraspan family of cell surface molecules that apparantly are involved in signal transduction or transporter system complexes. CD37 is strongly expressed on B lymphocytes (sIg-positive) and weakly on T cells, neutrophils and monocytes. Expression of CD37 disappears during B cell development at the plasma cell stage. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured human Raji cells per tube were incubated 45 min on ice with 80ul of anti-CD37 antibody at a concentration of 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.46 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice