C2386-02M

CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1) (FITC)

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£620.00

SKU:: C2386-02M
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Murine transformed endothelioma cell line tEnd.
Klon:: MEC14.7
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Weitere Details:: The CD34 cell surface antigen is expressed by endothelial cells and by haematopoietic stem cells. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:2.5-1:5. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-82E1: IgG2a, Rat Negative Control (FITC) Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.