C2379-09B

CD27 (CD27 Antigen, CD27 Molecule, CD27L Receptor, S152, T-cell Activation Antigen CD27, T14, Tp55, Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7) (FITC)

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£518.00

SKU:: C2379-09B
Anwendung:: Flow Cytometry
Reinheit:: Purified by size-exclusion chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Human peripheral blood lymphocytes
Klon:: LT27
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Weitere Details:: CD27 is a transmembrane 55kD protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32kD form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 20ul labels 10e6 cells in 100ul suspension or whole blood Optimal working dilutions to be determined by researcher. Excitation Laser: blue (488 nm) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice