C2275-89D
CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (PE)
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£442.00
- SKU:
- C2275-89D
- Reinheit:
- Purified
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- IL-2-dependent BALB/c mouse helper T-cell
- Klon:
- 5K251 (7D4)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
- Weitere Details:
- The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail(2). CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested(1, 3, 4). It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells(5, 6). CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus(6, 7). While the antibody does not block the binding of IL-2 to CD25, when used in combination with C2275-82 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone(3, 8, 9). Applications: Suitable for use in Immunoprecipitation and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: ≤0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Versandbedingungen:
- Blue Ice