C2275-86B

CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor)

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£691.00

SKU:: C2275-86B
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: 4°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Human PHA blasts; day 3 of culture.
Klon:: 3H1147 (MEM-181)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Weitere Details:: IL-2 receptor is expressed by activated lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:50-1:200. 10ul labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Recommended secondary reagent: I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab Rb xMo Recommended Negative Controls: I1904-78R: Mouse IgG1 Negative Control Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.