C2269-55Q
CD19 (B Lymphocyte Antigen CD19, B4, Differentiation Antigen CD19, Leu 12, Leu-12, MGC12802) (PE)
Größe
£730.00
- SKU:
- C2269-55Q
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Human K562 cell line transfected with mouse CD19.
- Klon:
- 6D5
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R. Phycoerythrin (R-PE). Reconstitute with 1ml sterile ddH2O.
- Weitere Details:
- CD19 is a member of the immunoglobulin superfamily with 556aa and two immunoglobulin-like C2-type domains. As a cell surface protein, CD19 is known to form a complex with CD21, CD81 and CD225 in the membrane of mature B cells. A major function of CD19 is to assemble with the antigen receptor of B lymphocytes so as to decrease the threshold for antigen receptor dependent stimulation thus enhancing the specificity and sensitivity of B cells towards antigens. CD19 is an important protein for B cell antigen receptor signaling and regulation and also acts as a specialized adaptor protein for the amplification of Src family needed for this purpose. Regulation of growth of B cells is a major function of CD19 and its expression is confined to only B lymphocytes and follicular dendritic cells of the hematopoietic system. Increased expression of CD19 induces the production of auto-antibody thus giving an insight to the regulatory role of CD19 in autoimmunity. Defects in CD19 are a cause of hypogammaglobulinemia. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat-1:10; 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized rats were fused with cells of the P3X63.Ag8.653 myeloma cell line. Recommended Negative Control: I1904-82H1: IgG2a, Rat Negative Control (PE) Storage and Stability: Lyophilized powder may be stored at 4°C. Reconstitute with sterile buffer or dH2O. Aliquot and store at 4°C. Reconstituted product is stable for 12 months as an undiluted liquid. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Versandbedingungen:
- Blue Ice