C2267-30L
CD16 (Fc gamma RIII alpha, NK cells)
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£691.00
- SKU:
- C2267-30L
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Klon:
- 10B814 (DJ130c)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Weitere Details:
- CD16 is a 50-65kD cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. In addition, CD16B exists as two allelic variants NA1 and NA2. This antibody recognises all polymorphonuclear cells irrespective on their NA phenotype. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended for this purpose. Immunohistochemistry: Frozen sections Flow Cytometry: 1:50 -1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Human tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice