C2266-01T
CD15 (Alpha-(1,3)-fucosyltransferase, CD15, ELAM-1 ligand fucosyltransferase, ELFT, FCT3A, Fucosyltransferase 4, Fucosyltransferase IV, FucT-IV, Fuc-TIV, FUC-TIV, FUTIV, Galactoside 3-L-fucosyltransferase, LeX, SSEA-1)
Größe
£479.00
- SKU:
- C2266-01T
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human neutrophils
- Klon:
- AHN1.1
- Format:
- Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 500 mM potassium chloride, 150mM sodium chloride. No preservative added.
- Weitere Details:
- Human CD15 is expressed by neutrophils, eosinophils and monocytes. Antibodies in this cluster recognize a common terminal pentasaccharide found on cell surface glycoproteins and glycolipids. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml labels 5x10e5 cultured U-937 human tumor cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured U-937 human tumor cells were incubated 45 minutes on ice with 80ul of C2266-01T at 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.47 log10 fluorescent units when compared to a mouse IgM negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice