C2265-32P1
CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein)
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£940.00
- SKU:
- C2265-32P1
- Reinheit:
- Purified by Protein A affinity chromatography from tissue culture supernatant. Endotoxin: <0.01EU/ug
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Klon:
- 3H501 (TÜK4)
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Weitere Details:
- CD14 is a ~55 kD glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmons et al. 1989), a soluble form of CD14 also exists (Bazil et al. 1986). CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria. C2265-32P1 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yang et al. 2003). Applications: Suitable for use in ELISA, Flow Cytometry, Functional Assays, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice