C2262-55F
CD11c (CD11 Antigen-Like Family Member C, CD11c, CD11C, Integrin alpha-X, Leukocyte Adhesion Glycoprotein p150,95 alpha Chain, Leukocyte Adhesion Receptor p150,95, Leu M5, SLEB6)
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£479.00
- SKU:
- C2262-55F
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human rheumatoid synovial fluid cells/monocytes
- Klon:
- 3.9
- Format:
- Supplied as a liquid in 50 mM sodium phosphate pH 7.5, 100 mM potassium chloride, 150mM sodium chloride. No preservative added.
- Weitere Details:
- Human CD11c (aX integrin) complexes with CD18 (B2 integrin) to form the complement receptor type 4 (CR4) heterodimer which binds to fibrinogen and is involved with monocyte/granulocyte adhesion during inflammatory responses. CD11c expression is restricted to leukocytes mainly of myeloid lineage with highest expression on macrophages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells per tube were washed and pre-incubated 5 min with 20ul of 250ug/ml human IgG (To block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD11c antibody at 10ug/ml. Cells were washed twice and incubated with 50ul of Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 15% sub population of cells stained positive with a mean shift of 1.01 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice