C2258-94C-BIOTIN

CD8a (Ly2, T cell Co-receptor, Leu2, T8) (Biotin)

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£960.00

SKU:: C2258-94C-BIOTIN
Reinheit:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Mouse spleen cells.
Klon:: YTS105.18
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Weitere Details:: Fusion Partners: Spleen cells from an immunized DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line. Applications: Suitable for use in hc Immunohistochemistry, Flow Cytometry. Recommended Dilution: Flow Cytometry: 1:50-1:100. 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Goat Anti Rat IgG: HRP (mouse absorbed), F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) Recommended Negative Controls: Rat IgG2a Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.