C2256-23D
CD5 (Lymphocyte Antigen T1/Leu 1, p56 62, T cell Surface Glycoprotein CD5, T1) (APC)
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£531.00
- SKU:
- C2256-23D
- Reinheit:
- Purified
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Klon:
- B19.1
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with Allophycocyanin (APC).
- Weitere Details:
- CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells (1-3). It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.13, 14 Applications: • Identification and enumeration of CD5+ cells by flow cytometry 15 • Identification of CD4+ cells in acetone-fixed frozen sections 15 • Immunoprecipitation 15 • Activation of T cells in vitro 15 • Western blotting 15 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. r.
- Versandbedingungen:
- Blue Ice