C2256-23
CD5 (Lymphocyte antigen T1/Leu 1, p56 62, T cell surface glycoprotein CD5, T1)
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£364.00
- SKU:
- C2256-23
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Klon:
- B19.1
- Format:
- Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
- Weitere Details:
- CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells (1-3). It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.13, 14 Applications: • Identification and enumeration of CD5+ cells by flow cytometry 15 • Identification of CD4+ cells in acetone-fixed frozen sections 15 • Immunoprecipitation 15 • Activation of T cells in vitro 15 • Western blotting 15 Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice