C2256-12M-HRP
CD5 (CD5 Antigen, CD5 Molecule, LEU1, Lymphocyte Antigen CD5, Lymphocyte Antigen T1/Leu 1, Lymphocyte Glycoprotein T1/Leu1, p56 62, T cell Surface Glycoprotein CD5, T1) (HRP)
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£914.00
- SKU:
- C2256-12M-HRP
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Concanavilin A activated canine peripheral blood cells
- Klon:
- 10B1757 (YKIX322.3)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Weitere Details:
- CD5, a 67kD cell surface type 1 transmembrane glycoprotein also known as Lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huang et al. 2008). CD5 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994). In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Villiers 2002). Applications: Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from an immunized DA rat were fused with cells of rat Y3/Ag1.2.3 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice