C2255-11Q

CD4 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (FITC)

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£552.00

SKU:: C2255-11Q
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Klon:: CT-4
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Weitere Details:: CD4 is a type I transmembrane glycoprotein expressed on approximately 70% of thymocytes, 15% of spleen cells and 40% of peripheral blood lymphocytes, but <1% of bursal cells.1 This antibody inhibits both PHA- and Con A-induced proliferative responses of splenocytes, and PWM-induced IL-2 production.1 Also reacts with turkey CD4 as demonstrated by flow cytometry and immunoprecipitation/Western blotting.3 Applications: • Flow cytometry 1 • Immunohistochemistry (frozen sections) 2 • Immunoprecipitation 1 • Inhibition of mitogen-induced proliferation 1 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.