C2255-05B-ML650
CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4, L3T4, LY-4, Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (MaxLight 650)
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£837.00
- SKU:
- C2255-05B-ML650
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse CD4
- Klon:
- 10A3 (GK1.5)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Weitere Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Recognises mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivo and in vitro cell depletion of CD4+ T-cells. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with Sspleen cells from immunised Lewis rats. Recommended Negative Control: I1904-82V4 IgG2b, Rat Negative Control I1904-82V3 IgG2b, Rat Negative Control I1904-82V2 IgG2b, Rat Negative Control (Azide Free) I1904-82V1 IgG2b, Rat Negative Control (Biotin) Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice