CD1c (Cluster of differentiation antigen 1c, CD1c antigen, CD1c antigen c polypeptide, CD1c molecule, Cortical thymocyte antigen CD1c, Differentiation antigen CD1 alpha 3, R7, T cell surface glycoprotein CD1c)

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£800.00

SKU:: C2252-03K
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Ala19-Met302 (Phe300Ser) from human CD1c, expressed in NSO cells.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: CD1c (Cluster of differentiation antigen 1c) is a 43kD member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12kD B2-microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316aa type I transmembrane glycoprotein. It contains a 285aa extracellular domain (ECD) (aa18-302) plus a 10aa cytoplasmic tail. The ECD shows one Ig-like domain (aa203-296) that associates with B2-microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa327-333, a second shows an eight aa substitution for aa298-333, and a third shows a 50aa substitution for aa297-333. There appears to be no direct mouse counterpart to human CD1c. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml in lysates of Jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice