C2251-02P
CD1a (T-cell Surface Glycoprotein CD1a, T-cell Surface Antigen T6/Leu-6, hTa1 Thymocyte Antigen)
Größe
£479.00
- SKU:
- C2251-02P
- Anwendung:
- Flow Cytometry
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Jurkat tumor cells
- Klon:
- CB-T6
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride.
- Weitere Details:
- The human CD1 genetic locus consists of five closely related genes (1). Three of the gene products (CD1a, CD1b and CD1c) have been officially clustered using antibodies and are expressed on cortical thymocytes, a subset of B cells, and on most professional antigen presenting cells (2). CD1 molecules have a specialized lipid antigen-presenting function (3,4) and it is unlikely that they present conventional peptide antigens. CD1a is an important marker to help identify developmental pathways of dendritic cells (5). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured HPB-MLT per tube were washed and incubated 45 min on ice with 80ul of anti-CD1a antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.21 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice