C2097-26
Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal Aspartyl Peptidase)
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£774.00
- SKU:
- C2097-26
- Molekulargewicht:
- 42
- Reinheit:
- ≥98%
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a lyophilized powder from 2mM sodium phosphate, pH 6.5. Reconstitute with sterile ddH2O.
- Weitere Details:
- Cathepsin D is a protein that in humans is encoded by the CTSD gene. This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. Cathepsins are a group of intracellular proteolytic enzymes located within the lysosomes. On the basis of the specificity towards their substrates, the cathepsins have been assigned the names: Cathepsins A, B, C, D, E, G H and L. Cathepsin D (EC 3.4.23.5) has been found to be widely distributed in various tissues but spleen is a rich source of this enzyme. Cathepsins A and D work synergistically to hydrolyze protein substrates. Cathepsin D acts as a true proteinase on substrates such as denatured hemoglobin at the pH range of 2.8–3.0. In this respect it behaves enzymatically similar to pepsin. Source: Native Protein, from Human Plasma Molecular Weight: ~42kD Specific Activity: ~300units/mg. Unit Definition: One unit of activity is defined as the amount of enzyme which digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1/60 minutes at 37°C. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice