C2088-50H
CASP10 (Caspase-10, CASP-10, ICE-like Apoptotic Protease 4, Apoptotic Protease Mch-4, FAS-associated Death Domain Protein Interleukin-1B-converting Enzyme 2, FLICE2, Caspase-10 Subunit p23/17, Caspase-10 Subunit p12, MCH4)
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£837.00
- SKU:
- C2088-50H
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to aa1-228 from human Pro Caspase-10 expressed in E.coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- Caspase-10 is a 28-29kD member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on procaspases-3, 4, 6, 7, and 9. Human procaspase-10a is a 59kD, 521aa protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29kD mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice